Recombinant D. melanogaster Ubiquitin-conjugating enzyme E2-17 kDa(eff)
- Catalog Number: CDRPO-3110264
- Size: 20 μg; 100 μg; 1000 μg
Target Information
Species | Drosophila melanogaster |
Target Names | eff |
Alternative Names | eff; UbcD1; CG7425; Ubiquitin-conjugating enzyme E2-17 kDa; EC 2.3.2.23; E2 ubiquitin-conjugating enzyme D1; Protein effete; Ubiquitin carrier protein; Ubiquitin-protein ligase |
Expression Region | 1-147 |
Protein Families | Ubiquitin-conjugating enzyme family |
Function | Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Required for proper telomere behavior during cell divisions and possibly for ubiquitination of proteins involved in postmeiotic stages of spermatogenesis. Deletion mutations are lethal in homozygotes. |
Database Info | KEGG: Dmel_CG7425; STRING: 7227.FBpp0082477; UniGene: Dm.4048 |
Product Details
Product Type | Recombinant Protein |
Source | Yeast; E. coli; Baculovirus; Mammalian cell |
Target Protein Sequence | MALKRINKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNELARE WTRKYAM |
Protein Length | Full Length Protein |
Purity | >85% (SDS-PAGE) |
Tag Info | The following tags are available. N-terminal His-tagged Tag-Free |
Form | Lyophilized powder |
Buffer before Lyophilization | Tris/PBS-based buffer, 6% Trehalose, pH 8.0 |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Lead Time | Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times. |
Storage & Handling
Storage Condition | Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use. |
Shelf Life | The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. |
Handling | Repeated freezing and thawing are not recommended. |
Limitations | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week. |
For research use only. Not intended for any clinical use.