Recombinant D. melanogaster Tubulin gamma-2 chain(gammaTub37C)

Drosophila melanogaster

gammaTub37C

gammaTub37C; Tub37CD; TubG2; TubG37C; CG17566; Tubulin gamma-2 chain; Gamma-2-tubulin

1-457

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Embryonic mitotic spindle, female meiotic spindle.

Tubulin family

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. Required for oocyte activation and consequently for organization of the female meiotic spindle. Essential for centrosome organization and assembly of biastral mitotic spindles in embryos. Plays a role in stabilizing the augmin complex on the meiotic spindle.

KEGG: Dmel_CG17566; STRING: 7227.FBpp0304827; UniGene: Dm.469

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MPSEIITLQL GQCGNQIGFE FWKRLCLEHG ISPDGVLEDF ATDGQDRKDV FFYQADDNHY IPRAVLIDLE PRVINNIMTS PYSKLYNQEN VFLSKHGGGA GNNWASGFSQ GEKVQEEVFD ILDREADGSD SLEGFVLCHS IAGGTGSGMG SYVLERLSER FPKKLIQTYS VFPNQDEISD VVVQPYNSIL TLKRLTKCAD SVVVLDNTAL NRIATERLHI QTPTFTQINN LVSTIMSLST TTLRYPSYMN NNLIGLTASL IPTPQLHFLM TGYTPLMSDC ETKTSVRKTT VLDVMRRLLQ PKNMMVSALT DKQSRQCFVS ILNIIQGEVD PSQVHKSLQR IRERKLANFI PWGPASIQVA LPRSSPYVQS AHKVSGLMMA NHTGISSLFK RALAQYDKLR KRNAFLDNFR RESMFQDDLT ELDIARDTVD CLVQEYEAAT QIDYPQWSPA VEASKAG

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.