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Recombinant D. melanogaster Tubulin gamma-2 chain(gammaTub37C)

  • Catalog Number:  CDRPO-3110357
  • Size:   20 μg; 100 μg; 1000 μg

Target Information

Species

Drosophila melanogaster

Target Names

gammaTub37C

Alternative Names

gammaTub37C; Tub37CD; TubG2; TubG37C; CG17566; Tubulin gamma-2 chain; Gamma-2-tubulin

Expression Region

1-457

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Embryonic mitotic spindle, female meiotic spindle.

Protein Families

Tubulin family

Function

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. Required for oocyte activation and consequently for organization of the female meiotic spindle. Essential for centrosome organization and assembly of biastral mitotic spindles in embryos. Plays a role in stabilizing the augmin complex on the meiotic spindle.

Database Info

KEGG: Dmel_CG17566; STRING: 7227.FBpp0304827; UniGene: Dm.469

Product Details

Product Type

Recombinant Protein

Source

Yeast; E. coli; Baculovirus; Mammalian cell

Target Protein Sequence

MPSEIITLQL GQCGNQIGFE FWKRLCLEHG ISPDGVLEDF ATDGQDRKDV FFYQADDNHY IPRAVLIDLE PRVINNIMTS PYSKLYNQEN VFLSKHGGGA GNNWASGFSQ GEKVQEEVFD ILDREADGSD SLEGFVLCHS IAGGTGSGMG SYVLERLSER FPKKLIQTYS VFPNQDEISD VVVQPYNSIL TLKRLTKCAD SVVVLDNTAL NRIATERLHI QTPTFTQINN LVSTIMSLST TTLRYPSYMN NNLIGLTASL IPTPQLHFLM TGYTPLMSDC ETKTSVRKTT VLDVMRRLLQ PKNMMVSALT DKQSRQCFVS ILNIIQGEVD PSQVHKSLQR IRERKLANFI PWGPASIQVA LPRSSPYVQS AHKVSGLMMA NHTGISSLFK RALAQYDKLR KRNAFLDNFR RESMFQDDLT ELDIARDTVD CLVQEYEAAT QIDYPQWSPA VEASKAG

Protein Length

Full Length Protein

Purity

>85% (SDS-PAGE)

Tag Info

The following tags are available. N-terminal His-tagged Tag-Free

Form

Lyophilized powder

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Lead Time

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Storage & Handling

Storage Condition

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

Shelf Life

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Handling

Repeated freezing and thawing are not recommended.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.

For research use only. Not intended for any clinical use.