Recombinant D. melanogaster Succinate dehydrogenase assembly factor 2-B, mitochondrial (CG12895)

Drosophila melanogaster

CG12895

CG12895; mitochondrial; SDH assembly factor 2-B; SDHAF2-B

25-156

Mitochondrion matrix.

SDHAF2 family

Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit of the SDH catalytic dimer.

KEGG: Dmel_CG12895; STRING: 7227.FBpp0303465

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

ASNLDK TEYTTPGEIV DYDDPPHLPV PEYPVRPDEP LETRKQRLLY QSRKRGMLEN DLLLSTFVAK HLKDFNAEQT AEYDQLINGV SNDWDIFYWA TDTKPTPPQF DTEIMRLLKE HVKNHEKVQR IRQPDL

Cytoplasmic Domain

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.