Recombinant D. melanogaster Stress-activated protein kinase JNK(bsk)

Drosophila melanogaster

bsk

bsk; JNK; CG5680; Stress-activated protein kinase JNK; dJNK; EC 2.7.11.24; Protein basket

1-372

Nucleus. Cytoplasm.

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily

Responds to activation by environmental stress by phosphorylating a number of transcription factors, primarily components of AP-1 such as Jra and also the transcriptional repressor aop, and thus regulates transcriptional activity. Component of the immune response activated by bacterial infection, and is involved in wound healing and in dorsal closure, a morphogenetic movement during embryogenesis. Functions in the systematic response to wounding acting downstream of the Hayan-phenoloxidase PPO1 cascade. Exhibits cytoprotective activity in neuronal cells in response to wounding to the integument. Controls the expression of a phosphatase, puckered, at the edges of wounded epidermal tissue and in the dorsal epithelium during dorsal closure. Regulates the activity of SREBP in neurons and thereby the accumulation of lipids in glia. Plays a role in postively regulating the expression of DIP2 independently of AP-1, thereby ensuring proper axon guidance in mushroom bodies.

KEGG: Dmel_CG5680; STRING: 7227.FBpp0079676; UniGene: Dm.1448

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MTTAQHQHYT VEVGDTNFTI HSRYINLRPI GSGAQGIVCA AYDTITQQNV AIKKLSRPFQ NVTHAKRAYR EFKLMKLVNH KNIIGLLNAF TPQRNLEEFQ DVYLVMELMD ANLCQVIQMD LDHDRMSYLL YQMLCGIKHL HSAGIIHRDL KPSNIVVKAD CTLKILDFGL ARTAGTTFMM TPYVVTRYYR APEVILGMGY TENVDIWSVG CIMGEMIRGG VLFPGTDHID QWNKIIEQLG TPSPSFMQRL QPTVRNYVEN RPRYTGYSFD RLFPDGLFPN DNNQNSRRKA SDARNLLSKM LVIDPEQRIS VDEALKHEYI NVWYDAEEVD APAPEPYDHS VDEREHTVEQ WKELIYEEVM DYEAHNTNNR TR

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.