Recombinant D. melanogaster Single-stranded DNA-binding protein, mitochondrial(mtSSB)

Drosophila melanogaster

mtSSB

mtSSB; lopo; CG4337; Single-stranded DNA-binding protein; mitochondrial; Dm mtSSB; Mt-SSB; MtSSB; Protein low power

23-146

Mitochondrion.

Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). Required to maintain the copy number of mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA replication that stimulate activity of the gamma complex polymerase tam at the replication fork. Promotes tam activity largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate tam activity.

KEGG: Dmel_CG4337; STRING: 7227.FBpp0099503; UniGene: Dm.5334

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

ATTTTAAA PAKVEKTVNT VTILGRVGAD PQLRGSQEHP VVTFSVATHT NYKYENGDWA QRTDWHRVVV FKPNLRDTVL EYLKKGQRTM VQGKITYGEI TDQQGNQKTS TSIIADDVLF FRDANN

Full Length of Mature Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.