Recombinant D. melanogaster RNA 3'-terminal phosphate cyclase

Drosophila melanogaster

CG4061

CG4061; RNA 3'-terminal phosphate cyclase; RNA cyclase; RNA-3'-phosphate cyclase; EC 6.5.1.4

1-361

Nucleus, nucleoplasm.

RNA 3'-terminal cyclase family, Type 1 subfamily

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.

KEGG: Dmel_CG4061; STRING: 7227.FBpp0070316; UniGene: Dm.33463

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MDAEKMLEID GSYLEGGGQA LRNALSLSCI LGKPVRVVKI RASRPSPGLS HQHLHGLNLL RDITNADVVG NYLLSSTVEF TPRTILDNTY RVETHTAASI TLIYQMALPV LLFAGRPSRL IVSGGTNVDF APPVEYMQEV LLPNLKHFGV SFDLKVQRYG FYPRGQGRCQ LDVQPVTKLN SGKLVAFGRI KSVSGVAYCA GRLPVNIAID MQQTAQREIH RLWPSQQCSI EPIKHSRQKA FHNGAGILMT VNTTSDVVLG ASALGKKRID GHVLGSEASC QLGDYMRKQV CVDDYMQDQL IIYMALAVGR STMRTGKLTN HTRTAINVAE QMTGVKFDVA MEPGGQMLVS CKGLGHVNKL I

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.