Recombinant D. melanogaster Ras-related protein Rac1(Rac1)

Drosophila melanogaster

Rac1

Rac1; RacA; CG2248; Ras-related protein Rac1

1-189

Cell membrane; Lipid-anchor; Cytoplasmic side.

Small GTPase superfamily, Rho family

During various developmental processes, regulates changes in cell morphology in response to extracellular signals. During oogenesis, mediates signaling from the tyrosine kinase (RTK) chemoattractant receptors (Egfr and Pvr) to the guidance pathway that control the directional persistent collective migration of the border cell (BC) cluster through the nurse cells to the oocyte. Once activating by Pvr and Egfr, promotes the formation of forward-directed actin protrusions which stabilize the DE-cadherin (shg)-mediated adhesions. In turn, DE-mediated adhesion between the leader border cells and nurse cells further promotes Rac1 signaling creating a positive feedback loop that amplifies the output of RTK activity and leads to higher Rac activity at the front, thus promoting polarization of the border cell cluster and directionally persistent migration. Involved in axon outgrowth and myoblast fusion. Plays a role in regulating dorsal closure during embryogenesis. Involved in integrin alpha-PS3/beta-nu-mediated phagocytosis of Gram-positive S.aureus by hemocytes.

KEGG: Dmel_CG2248; STRING: 7227.FBpp0304252; UniGene: Dm.4299

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDAKP INLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVNPASF ENVRAKWYPE VRHHCPSTPI ILVGTKLDLR DDKNTIEKLR DKKLAPITYP QGLAMAKEIG AVKYLECSAL TQKGLKTVFD EAIRSVLCPV LQPKSKRKC

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.