Recombinant D. melanogaster Putative serine/threonine-protein kinase haspin homolog(Haspin), partial

Drosophila melanogaster

Haspin

Haspin; CG40080; Putative serine/threonine-protein kinase haspin homolog; EC 2.7.11.1

Nucleus lamina. Chromosome. Cytoplasm, cytoskeleton, spindle.

Protein kinase superfamily, Ser/Thr protein kinase family, Haspin subfamily

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-4' (H3T3ph) during mitosis and interphase. Function is essential for chromosome organization during mitosis and genome organization in interphase cells, thus playing a functional role in gene regulation. During mitosis, may act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle. During interphase, associates with the cohesion complex and mediates pds5 binding to chromatin to ensure correct sister chromatid cohesion, chromatin organization, and also functions with Pds5-cohesin to modify Polycomb-dependent homeotic transformations. Function during interphase is required for insulator activity, nuclear compaction, heterochromatin-induced position-effect variegation and PcG-mediated pairing-sensitive silencing.

KEGG: Dmel_CG40080; STRING: 7227.FBpp0112507; UniGene: Dm.28538

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

Partial

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.