Recombinant D. melanogaster Protein Tube(tub)

Drosophila melanogaster

tub

tub; tube; CG10520; Protein Tube

1-462

Cytoplasm. Cell membrane. Note=Associates with the plasma membrane during interphase syncytial blastoderm embryos, more specifically at the membrane invaginations around the nuclei. Later in embryonic development protein is entirely cytoplasmic.

Plays an essential role in the Tl receptor signaling pathway that establishes embryonic dorsoventral polarity; the signal directs import of dl into ventral and ventrolateral nuclei, thereby establishing dorsoventral polarity. Tub recruits pll to the plasma membrane and protein-protein interaction activates pll. Also has a role in pupal pattern formation.

KEGG: Dmel_CG10520; STRING: 7227.FBpp0271525; UniGene: Dm.2494

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MAYGWNGCGM GVQVNGSNGA IGLSSKYSRN TELRRVEDND IYRLAKILDE NSCWRKLMSI IPKGMDVQAC SGAGCLNFPA EIKKGFKYTA QDVFQIDEAA NRLPPDQSKS QMMIDEWKTS GKLNERPTVG VLLQLLVQAE LFSAADFVAL DFLNESTPAR PVDGPGALIS LELLEEEMEV DNEGLSLKYQ SSTATLGADA QGSVGLNLDN FEKDIVRRDK SVPQPSGNTP PIAPPRRQQR STTNSNFATL TGTGTTSTTI PNVPNLTILN PSEQIQEPVL QPRPMNIPDL SILISNSGDL RATVSDNPSN RTSSTDPPNI PRITLLIDNS GDVNSRPNHA PAKASTATTS TASSNNLPMI SALNISKGSR ETLRPESRSS SSSLSKDDDD DNDGEEDGEE EYPDAFLPNL SNSEQQSSNN DSSLTTVTGT SGDNSFELTN DSSSTSNDDY ACNIPDLSEL QQ

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.