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Recombinant D. melanogaster Protein timeless(tim), partial

  • Catalog Number:  CDRPO-3110912
  • Size:   20 μg; 100 μg; 1000 μg

Target Information

Species

Drosophila melanogaster

Target Names

tim

Alternative Names

tim; CG3234; Protein timeless

Subcellular Location

Nucleus. Cytoplasm, perinuclear region. Note=Nuclear at specific periods of the day. First accumulates in the perinuclear region about one hour before translocation into the nucleus. Interaction with per is required for nuclear localization.

Protein Families

Timeless family

Function

Required for the production of circadian rhythms. The biological cycle depends on the rhythmic formation and nuclear localization of the TIM-PER complex. Light induces the degradation of TIM, which promotes elimination of PER. Nuclear activity of the heterodimer coordinatively regulates PER and TIM transcription through a negative feedback loop. Behaves as a negative element in circadian transcriptional loop. Does not appear to bind DNA, suggesting indirect transcriptional inhibition.

Database Info

KEGG: Dmel_CG3234; STRING: 7227.FBpp0305454; UniGene: Dm.2663

Product Details

Product Type

Recombinant Protein

Source

Yeast; E. coli; Baculovirus; Mammalian cell

Protein Length

Partial

Purity

>85% (SDS-PAGE)

Tag Info

The following tags are available. N-terminal His-tagged Tag-Free

Form

Lyophilized powder

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Lead Time

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Storage & Handling

Storage Condition

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

Shelf Life

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Handling

Repeated freezing and thawing are not recommended.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.

For research use only. Not intended for any clinical use.