Recombinant D. melanogaster Protein SMG9 (CG3857)

Drosophila melanogaster

CG3857

CG3857; Protein SMG9; Protein smg-9 homolog

1-487

SMG9 family

Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Probable component of kinase complex containing nonC and recruited to stalled ribosomes.

KEGG: Dmel_CG3857; STRING: 7227.FBpp0070335; UniGene: Dm.29134

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MADPRRRFRN KKRDEACSGL LAPVTIARRE DAARMMQPKI LLKKDRDREQ ETWDRERDKD RKLERDREAE PSPSCYPDTP PALKTMIVNR TGEVRPADRC QMPLAGGALV QGSGQLSAVP SSSVCAALVS SVPTSRDKGS CSGGAGTAGT SAGAPNALQE LQPPRMNRPT PLIVANGIFN ANARKLFHKT NTDFTVIGVL GGQSSGKSTL LNLLAAERSL DYDYYQHLFS PEADECIFAT RHKLKPNNGQ KSILRPRTET LQFFITRERH ILLDTPPLMP VGKDSDHQDL YSLGTMAQLL SVCHILILVI DGLALEQLRL INAALRLRPT LHCKGYVRDH MPQVVFVRAR AHRIDFEIQQ RERLDKKLAY LYGPTGLPIY RGRGDARCLN TFLLPEVSSN KATAFHSCLG ELVRQFRERI LGCTRISMCH TSTELSEAIW FEILAESARK AAPHFEKIYA EIKLRHLDTR CQWRSDNWRT FSSNAES

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.