Recombinant D. melanogaster Protein Shroom(Shroom), partial

Drosophila melanogaster

Shroom

Shrm; Shroom; CG34379

[Isoform D]: Cell junction, adherens junction. Cytoplasm, cytoskeleton; [Isoform F]: Apical cell membrane; Peripheral membrane protein.

Shroom family

Binds to Rho-kinase Rok and targets it to the apical cell cortex where it mediates apical constriction. During embryogenic axis elongation, required for the localization to adherens junctions and the establishment of planar polarization of both Rho-kinase Rok and myosin regulatory light chain sqh. May be involved in the assembly of microtubule arrays during cell elongation.

KEGG: Dmel_CG34379; UniGene: Dm.2231

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

Partial

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.