Recombinant D. melanogaster Protein nanos(nos)

Drosophila melanogaster

nos

nos; CG5637; Protein nanos

1-401

Cytoplasm, Cytoplasmic ribonucleoprotein granule.

Nanos family

Maternal RNA-binding protein that is required for germ cells proliferation and self-renewal. Acts by forming a complex with pum and brat that regulates translation and mRNA stability. The complex binds to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR and prevents its translation. Controls posterior development. Rescuing factor for the abdominal defect of posterior group mutants. The other posterior group genes are not required for nanos function but rather play a role in localization or distribution of nanos protein.

KEGG: Dmel_CG5637; STRING: 7227.FBpp0083146; UniGene: Dm.1485

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MFRSNLEGSG AAAVGVANPP SLAQSGKIFQ LQDNFSAFHA RGGLNILGLQ DMYLDTSGAN SSATLSPPIT PVTPDPSTSA QSTHFPFLAD SAATANSLLM QRQYHYHLLL QQQQQLAMAQ HQLALAASAA AASASHQQTD EIARSLKIFA QVTTGAAENA AGSMQDVMQE FATNGYASDD LGRMSYGSAP PQVQMPPQQQ HQQQQGLHLP LGRNPAQLQT NGGNLMPIPL ATHWLNNYRE HLNNVWRNMS YIPAAPNTMG LQAQTAATVS TNLGVGMGLG LPVQGEQLRG ASNSSNNNNN NNKVYKRYNS KAKEISRHCV FCENNNEPEA VINSHSVRDN FNRVLCPKLR TYVCPICGAS GDSAHTIKYC PKKPIITMED AIKAESFRLA KSSYYKQQMK V

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.