Recombinant D. melanogaster Protein dimmed(dimm)

Drosophila melanogaster

dimm

dimm; CG8667

1-390

Cytoplasm.

Transcription factor that regulates neurosecretory (NS) cell function and neuroendocrine cell fate. Acts as a master regulator of common NS functions such as Phm expression and neuropeptide production. Plays a role as a regulator of peptide-containing large dense-core vesicle (LDCV) production and peptidergic cell differentiation. Controls transcription of FMRFamide in Tv neuronal cells and Fur1 in Ap-let cells (Tvb and dorsal apterous cells). Also required for up-regulation of Phm in Tv and Ap-let cells, and expression of three neuropeptide genes, Ms, FMRFamide and Lk. Influences both regulated and constitutive secretory activity in neuroendocrine cells at embryonic and postembryonic level. Loss of function studies show reduced cellular levels of various neuropeptides and neuropeptide biosynthetic enzymes.

KEGG: Dmel_CG8667; STRING: 7227.FBpp0305546; UniGene: Dm.23895

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MDATQLTELM GSHDFMQLQH QLHHNNNNYN TDGHNGLSSE SAEGSSRPVR RATRRTSQLS NNTYDLEMTD SSSQSDDTSG GGGSSNGGGS TTNTGHPSGC SLGGQGPSGR GRVQQASSGA CPSTIAPNST SSNSSNANGN ASRRRKGALN AKERNMRRLE SNERERMRMH SLNDAFQSLR EVIPHVEMER RLSKIETLTL AKNYIINLTH IILSKRNEEA AALELNSGAV GGVLLSNLSS ESGGPVASGI PANSNAATIC FEDTLASGGA FDCAILAATD GSLLNAATVT TSPAMQSIQS QAIHLQTPME QQQQQASHLP HHQQAMHGHG HLGASIQSQQ QPSLVLNGTT SVGLGIGIGV GVGVGVGVCN NAPSFADIND NFDEPFREFL

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.