Recombinant D. melanogaster Protein crumbs(crb), partial

Drosophila melanogaster

crb

crb; CG6383; 95F

Apical cell membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton, spindle.

Crumbs protein family

Plays a central role in cell polarity establishment, and contributes to the organization of zonula adherens, epithelial morphogenesis, and tissue growth. Participates in the assembly, positioning and maintenance of adherens junctions via its interaction with the SAC complex. Controls the coalescence of the spots of zonula adherens (ZA) into an adhesive ring around the cells. It may act as a signal. Involved in morphogenesis of the photoreceptor rhabdomere, for positioning and growth of rhabdomere and AJ during the crucial period of photoreceptor extension along the proximodistal axis of the retina. In cooperation with aPKC, required for the formation and maintenance of the rhabdomeric and stalk apical cortical membrane domains. Recruits aPKC to the apical stalk membrane where it phosphorylates and displaces rhabdomere specifying proteins such as PIP82, to restrict their localization and thus activity to the rhabdomeric apical domain. Component of the crb-galla-Xpd (CGX) complex which is essential for proper mitotic chromosome segregation in early embryos. The CGX complex is also required for cell proliferation in developing wing disks. In the CGX complex, acts with galla-1 or galla-2 to recruit Xpd and thus form the functional complex. Together with apn, plays a key role in trachea development at larval stages.

KEGG: Dmel_CG6383; STRING: 7227.FBpp0293268; UniGene: Dm.2365

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

Partial

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.