Recombinant D. melanogaster Probable ubiquitin carboxyl-terminal hydrolase FAF(faf), partial

Drosophila melanogaster

faf

faf; CG1945; EC 3.4.19.12; Protein fat facets; Ubiquitin thioesterase FAF; Ubiquitin-specific-processing protease FAF; Deubiquitinating enzyme FAF

Peptidase C19 family

Ubiquitin C-terminal hydrolase involved in development and the imd/NF-kappa-B (IMD) signaling cascade. Required for eye and embryo development, and plays a role in compound eye assembly and oogenesis respectively. In the larval eye disks, cells outside the assembling facets require this protein for short-range cell interactions that prevent the mystery cells from becoming photoreceptors. Also required for nuclear migration and cellularization in early embryogenesis and could play a role in pole cell determination, development or function. Regulates the IMD signaling cascade at later stages of infection (around 6 hours post-infection) by inhibiting the expression of the antimicrobial peptides Dpt and Dro. Acts by modulating the state of imd polyubiquitination and/or stability; a function which appears to be independent of its enzymatic activity. In turn, imd enhances the polyubiquitination and stability of faf suggesting that they may form a regulatory feedback mechanism within the Imd pathway.

KEGG: Dmel_CG1945; STRING: 7227.FBpp0085202; UniGene: Dm.3133

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

Partial

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.