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Recombinant D. melanogaster Probable phenylalanine--tRNA ligase, mitochondrial(Aats-phe)

  • Catalog Number:  CDRPO-3110654
  • Size:   20 μg; 100 μg; 1000 μg

Target Information

Species

Drosophila melanogaster

Target Names

PheRS-m

Alternative Names

PheRS-m; Aats-phe; Aats-phe-m; Pts; CG13348; Probable phenylalanine--tRNA ligase; mitochondrial; EC 6.1.1.20; Phenylalanyl-tRNA synthetase; PheRS

Expression Region

28-453

Subcellular Location

Mitochondrion matrix.

Protein Families

Class-II aminoacyl-tRNA synthetase family

Function

Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation.

Database Info

KEGG: Dmel_CG13348; STRING: 7227.FBpp0086720; UniGene: Dm.4156

Product Details

Product Type

Recombinant Protein

Source

Yeast; E. coli; Baculovirus; Mammalian cell

Target Protein Sequence

KSP SSPPQLEVSG STYATDGWTN VTPKILSYVG ANKHLQTDHP LSIIRQRIVN YFYGAYRNQR GNPLFSVYDQ MNPVVTVQQN FDNLLIPADH VSRQKSDCYY INQQHLLRAH TTAHQVELIS GGLDNFLVVG EVYRRDEIDS THYPVFHQAD AVRLVTKDKL FERNPGLELF EETWSGTLAD PKLILPSSKF MDQTKQPCHT LEAVKLMEHE MKHVLVGLTK DLFGPRIKYR WVDTYFPFTQ PSWELEIYFK DNWLEVLGCG IMRHEILQRS GVHQSIGYAF GVGLERLAMV LFDIPDIRLF WSNDSGFLSQ FSEKDLHNLP KYKPISHYPQ CTNDLSFWLP QDIEVDAGFS PNDFYDLVRS VAGDMVEQIS LVDKFKHPKT GKSSVCFRIV YRHMERTLTQ AEVNEIHKQI ASASVDSFNV QIR

Protein Length

Full Length of Mature Protein

Purity

>85% (SDS-PAGE)

Tag Info

The following tags are available. N-terminal His-tagged Tag-Free

Form

Lyophilized powder

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Lead Time

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Storage & Handling

Storage Condition

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

Shelf Life

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Handling

Repeated freezing and thawing are not recommended.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.

For research use only. Not intended for any clinical use.