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Recombinant D. melanogaster POU domain protein CF1A(vvl)

  • Catalog Number:  CDRPO-3110972
  • Size:   20 μg; 100 μg; 1000 μg

Target Information

Species

Drosophila melanogaster

Target Names

vvl

Alternative Names

vvl; Cf1a; Chorion factor 1A; CF1-A; Protein drifter; Ventral veins lacking protein

Expression Region

1-427

Subcellular Location

Nucleus.

Protein Families

POU transcription factor family, Class-3 subfamily

Function

Binds to a DNA sequence element required for the expression of the dopa decarboxylase gene (Ddc) in specific dopaminergic neurons. Could also play an early role in specific ectodermal cells, and a subsequent role in the embryonic nervous system.

Database Info

KEGG: Dmel_CG10037; STRING: 7227.FBpp0303092; UniGene: Dm.2556

Product Details

Product Type

Recombinant Protein

Source

Yeast; E. coli; Baculovirus; Mammalian cell

Target Protein Sequence

MAATSYMTPP SGDLDMALGG GGYHTSSPRS AADAGEMKYM QHHHHHHAAA AAAAHHQLPS SPSPNGQGNG GGLGLGSGSG LGSWSALHPD PWMQTHHTHH LPAAAAVASA ADTVKQEMSH LSQQTRIQQG MASPHAAWHA PHAGHYAPTG GSPLQYHHAM NGMLHHPAHA VAAAHHQSVA PLHHTLRGES PQLHIHHHMG GGDRDAISGG EEDTPTSDDL EAFAKQFKQR RIKLGFTQAD VGLALGTLYG NVFSQTTICR FEALQLSFKN MCKLKPLLQK WLEEADSTTG SPTSIDKIAA QGRKRKKRTS IEVSVKGALE QHFHKQPKPS AQEITSLADS LQLEKEVVRV WFCNRRQKEK RMTPPNTLGG DMMDGMPPGH MHHGGYHPHH DMHGSPMGTH SHSHSPPMLS PQNMQSSAVA AHQLAAH

Protein Length

Full Length Protein

Purity

>85% (SDS-PAGE)

Tag Info

The following tags are available. N-terminal His-tagged Tag-Free

Form

Lyophilized powder

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Lead Time

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Storage & Handling

Storage Condition

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

Shelf Life

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Handling

Repeated freezing and thawing are not recommended.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.

For research use only. Not intended for any clinical use.