Recombinant D. melanogaster Peptidylglycine alpha-hydroxylating monooxygenase(Phm)
- Catalog Number: CDRPO-3110656
- Size: 20 μg; 100 μg; 1000 μg
Target Information
| Species | Drosophila melanogaster |
| Target Names | Phm |
| Alternative Names | Phm; CG3832; Peptidylglycine alpha-hydroxylating monooxygenase; dPHM; EC 1.14.17.3 |
| Expression Region | 25-365 |
| Subcellular Location | Secreted. |
| Protein Families | Copper type II ascorbate-dependent monooxygenase family |
| Function | Monooxygenase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Produces an unstable peptidyl(2-hydroxyglycine) intermediate. C-terminal amidation of peptides is required for normal developmental transitions and for biosynthesis of secretory peptides throughout the life. |
| Database Info | KEGG: Dmel_CG3832; STRING: 7227.FBpp0072112; UniGene: Dm.2282 |
Product Details
| Product Type | Recombinant Protein |
| Source | Yeast; E. coli; Baculovirus; Mammalian cell |
| Target Protein Sequence | LVKEGD YQNSLYQQNL ESNSATGATA SFPFLMPNVS PQTPDLYLCT PIKVDPTTTY YIVGFNPNAT MNTAHHMLLY GCGEPGTSKT TWNCGEMNRA SQEESASPCG PHSNSQIVYA WARDAQKLNL PEGVGFKVGK NSPIKYLVLQ VHYAHIDKFK DGSTDDSGVF LDYTEEPRKK LAGTLLLGTD GQIPAMKTEH LETACEVNEQ KVLHPFAYRV HTHGLGKVVS GYRVRTNSDG EQEWLQLGKR DPLTPQMFYN TSNTDPIIEG DKIAVRCTMQ STRHRTTKIG PTNEDEMCNF YLMYYVDHGE TLNMKFCFSQ GAPYYFWSNP DSGLHNIPHI EASTL |
| Protein Length | Full Length of Mature Protein |
| Purity | >85% (SDS-PAGE) |
| Tag Info | The following tags are available. N-terminal His-tagged Tag-Free |
| Form | Lyophilized powder |
| Buffer before Lyophilization | Tris/PBS-based buffer, 6% Trehalose, pH 8.0 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Lead Time | Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times. |
Storage & Handling
| Storage Condition | Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use. |
| Shelf Life | The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. |
| Handling | Repeated freezing and thawing are not recommended. |
| Limitations | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week. |
For research use only. Not intended for any clinical use.