Recombinant D. melanogaster Nucleoside diphosphate kinase(awd)

Drosophila melanogaster

awd

awd; K-pn; CG2210; Nucleoside diphosphate kinase; NDK; NDP kinase; EC 2.7.4.6; Abnormal wing disks protein; Killer of prune protein

2-153

Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.

NDK family

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.

KEGG: Dmel_CG2210; STRING: 7227.FBpp0085223; UniGene: Dm.6904

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

AANKERTFI MVKPDGVQRG LVGKIIERFE QKGFKLVALK FTWASKELLE KHYADLSARP FFPGLVNYMN SGPVVPMVWE GLNVVKTGRQ MLGATNPADS LPGTIRGDFC IQVGRNIIHG SDAVESAEKE IALWFNEKEL VTWTPAAKDW IYE

Full Length of Mature Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.