Recombinant D. melanogaster Inorganic pyrophosphatase(Nurf-38)

Drosophila melanogaster

Nurf-38

Nurf-38; CG4634; Inorganic pyrophosphatase; EC 3.6.1.1; Nucleosome-remodeling factor 38 kDa subunit; Pyrophosphate phospho-hydrolase; PPase

1-338

Cytoplasm. Nucleus.

PPase family

Component of NURF (nucleosome remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. NURF is required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis. Nurf-38 may have adapted to deliver pyrophosphatase to chromatin to assist in replication or transcription by efficient removal of the inhibitory metabolite.

KEGG: Dmel_CG4634; STRING: 7227.FBpp0072250; UniGene: Dm.1958

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MLAKITRSSF YASRAVGRLS GSIPTSPAAL ASNCRYIQIE RKRTKSHEMA LYETVEKGAK NSPSYSLYFK NKCGNVISPM HDIPLYANEE KTIYNMVVEV PRWTNAKMEI SLKTPMNPIK QDIKKGKLRF VANCFPHKGY IWNYGALPQT WENPDHIEPS TGCKGDNDPI DVIEIGYRVA KRGDVLKVKV LGTIALIDEG ETDWKIIAID VNDPLASKVN DIADVDQYFP GLLRATVEWF KIYKIPDGKP ENQFAFNGDA KNADFANTII AETHKFWQNL VHQSPASGSI STTNITNRNS EHVIPKEEAE KILAEAPDGG QVEEVSDTVD TWHFIHLK

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.