Recombinant D. melanogaster Hsc70-interacting protein 1(HIP)

Drosophila melanogaster

HIP

HIP; CG32789

1-377

Cytoplasm.

FAM10 family

One HIP oligomer binds the ATPase domains of at least two Hsc70 molecules dependent on activation of the Hsc70 ATPase by Hsp40. Stabilizes the ADP state of Hsc70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of Hsc70 with various target proteins.

KEGG: Dmel_CG2947; STRING: 7227.FBpp0070575; UniGene: Dm.33464

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MAFTMQTGDL KKLKYFIDFA LENPTFLNMP QLQFVKDFVE KFGGTVPPGQ FNGGSAGGKC PFGGVAGAKA NEPANAPEDS EDEKSLSDPE SDVELDMEGV IEADSDPAQP MGNYSKKATE EEVEQASELR AQAASAYGQQ KFDEAIALYT KAIELSPGNA LFHAKRGQAF LKLKKPNACI RDCDVALELN SDLAAGYKFR GRARRLLGDF ELAAHDLRQA CKLDFDEETD EWLKEVTPNA KKIEQHRLKQ ERRQAERKIK ERQRDQRRAR KEQEKHNASS GGSSGEFSGG NPGNGNMSDI LGAMSDPEVS AAIQDILSNP GNITKYASNP KIYNLIKKIV PGGDVGAAFG QAGEKAGKPS EPKPKKDSAD FVDDGLD

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.