Recombinant D. melanogaster Homeotic protein caudal(cad)

Drosophila melanogaster

cad

cad; CG1759

1-427

Nucleus.

Caudal homeobox family

Caudal (cad) is one of a number of transcription factors controlling segmentation of the embryo. Further transcriptional regulation via a 5' flanking region containing DNA replication-related elements (DRE) and by dref also regulated by trh and tgo via the CNS midline element. Alongside Bicoid (bcd), caudal forms concentration gradients down the anterior-posterior (A-P) axis providing positional information and subsequent induction of the gap genes. Plays a role in gastrulation/germ band extension, hindgut morphogenesis, positive regulation of cell proliferation, genital disk development and pattern formation. Acts as a key regulator of the Hox gene network and activates transcription via the downstream core promoter element (DPE) relative to the TATA box. Plays a role in the establishment of the hindgut and in the invagination of the hindgut primordium during gastrulation. These effects on the gut are achieved by acting combinatorially at the posterior of the embryo to activate transcription of different targets including fog, fkh and wg. Caudal is involved in regulation of proliferation through transactivation of the E2F gene. Postembryonically its function is mostly restricted to the intestine where it regulates antimicrobial peptide (AMP) levels preserving the normal gut flora.

KEGG: Dmel_CG1759; STRING: 7227.FBpp0080942; UniGene: Dm.2073

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MVSHYYNTLP YTQKHSAANL AYASAAGQPW NWTPNYHHTP PNHQFLGDVD SSHAAHHAAA AHQMYYNSHH MFHSAAAASA GEWHSPASST ADNFVQNVPT SAHQLMQQHH HHHAHASSSS ASSGSSSSGG APGAPQLNET NSSIGVGGAG GGGGVGGATD GGPGSAPPNH QQHIAEGLPS PPITVSGSEI SSPGAPTSAS SPHHHLAHHL SAVANNNNNN NNNNNSPSTH NNNNNNNSVS NNNRTSPSKP PYFDWMKKPA YPAQPQPGKT RTKDKYRVVY TDFQRLELEK EYCTSRYITI RRKSELAQTL SLSERQVKIW FQNRRAKERK QNKKGSDPNV MGVGVQHADY SQLLDAKAKL EPGLHLSHSL AHSMNPMAAM NIPAMRLHPH LAAHSHSLAA VAAHSHQLQQ QHSAQMSAAA AVGTLSM

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.