Recombinant D. melanogaster Guanine nucleotide-binding protein G(i) subunit alpha 65A

Drosophila melanogaster

Galphai

Galphai; G-ialpha65A; G-OA65C; Guanine nucleotide-binding protein G(i) subunit alpha 65A

2-355

Cell membrane. Apical cell membrane. Note=Colocalizes with loco and raps/pins at the apical cortex throughout mitosis in neuroblasts.

G-alpha family, G(i/o/t/z) subfamily

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Plays a role in glial cell differentiation during embryogenesis; loco, Galphao and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord.

KEGG: Dmel_CG10060; STRING: 7227.FBpp0076643; UniGene: Dm.33467

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

GCAVSTARD KEAIERSKNI DRALRAEGER AASEVKLLLL GAGESGKSTI VKQMKIIHDT GYSQEECEEY RRVVFSNTVQ SLMVIIRAMG RLKIEFADPS RTDIARQFFT HASAADEGIL LPEIVLLMKK LWADGGVQQS FARSREYQLN DSAGYYLNSL DRIAQPNYIP TQQDVLRTRV KTTGIIETHF SCKQLHFKLF DVGGQRSERK KWIHCFEGVT AIIFCVALSG YDLVLAEDEE MNRMIESLKL FDSICNSKWF VETSIILFLN KKDLFEEKIK RSPLTICFPE YTGTNTFEEA ANYIRMKFEN LNKRKDQKEI YTHLTCATDT NNVKFVFDAV TDVIIKNNLK QIGLF

Full Length of Mature Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.