Recombinant D. melanogaster General transcription factor IIF subunit 2(TfIIFbeta)
- Catalog Number: CDRPO-3110908
- Size: 20 μg; 100 μg; 1000 μg
Target Information
| Species | Drosophila melanogaster |
| Target Names | TfIIFbeta |
| Alternative Names | TfIIFbeta; TFIIF30; CG6538; General transcription factor IIF subunit 2; EC 3.6.4.12; ATP-dependent helicase TfIIF-beta; Transcription initiation factor IIF subunit beta; TFIIF-beta |
| Expression Region | 1-277 |
| Subcellular Location | Nucleus. |
| Protein Families | TFIIF beta subunit family |
| Function | TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity. |
| Database Info | KEGG: Dmel_CG6538; STRING: 7227.FBpp0081786; UniGene: Dm.20167 |
Product Details
| Product Type | Recombinant Protein |
| Source | Yeast; E. coli; Baculovirus; Mammalian cell |
| Target Protein Sequence | MSKEDKEKTQ IIDKDLDLSN AGRGVWLVKV PKYIAQKWEK APTNMDVGKL RINKTPGQKA QVSLSLTPAV LALDPEEKIP TEHILDVSQV TKQTLGVFSH MAPSDGKENS TTSAAQPDNE KLYMEGRIVQ KLECRPIADN CYMKLKLESI RKASEPQRRV QPIDKIVQNF KPVKDHAHNI EYRERKKAEG KKARDDKNAV MDMLFHAFEK HQYYNIKDLV KITNQPISYL KEILKDVCDY NMKNPHKNMW ELKKEYRHYK TEEKKEEEHK SGSSDSE |
| Protein Length | Full Length Protein |
| Purity | >85% (SDS-PAGE) |
| Tag Info | The following tags are available. N-terminal His-tagged Tag-Free |
| Form | Lyophilized powder |
| Buffer before Lyophilization | Tris/PBS-based buffer, 6% Trehalose, pH 8.0 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Lead Time | Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times. |
Storage & Handling
| Storage Condition | Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use. |
| Shelf Life | The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. |
| Handling | Repeated freezing and thawing are not recommended. |
| Limitations | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week. |
For research use only. Not intended for any clinical use.