Recombinant D. melanogaster Eukaryotic translation initiation factor 5(eIF5)

Drosophila melanogaster

eIF5

eIF5; CG9177; Eukaryotic translation initiation factor 5; eIF-5

1-464

EIF-2-beta/eIF-5 family

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

KEGG: Dmel_CG9177; STRING: 7227.FBpp0073947; UniGene: Dm.173

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MATVNVNRSV TDIFYRYKMP RLQAKVEGKG NGIKTVLVNM AEVARAIGRP ATYPTKYFGC ELGAQTLFDH KNERFVVNGS HDVNKLQDLL DGFIRKFVLC PECDNPETNL TVSAKNQTIS QSCKACGFHG LLKVNHKVNT FIVKNPPSLN PAAQGSSLTE GKRSRKQKQK NDNADGSMTN NSLANNSGGE SDGGNGTNQA SQTEAEISAA IPEKTAKDDD DEGWSVDVSK EAIRARLQDL TDGAKGMTIS DDYDKTEKER IDIFYELVKD KRDKKQLDDV QTHKELVIEA ERLDIINKAP LVLAELLFTE NIIKDVQKNR PLLLRFTLNN PKAQRYLIGG VEQTVELHKG ILMSKVAGIF KLFYDLDILD EAVILEWAQK VSKRHVSKNI AAEIHEKAMP FVLWLKNAEE ESSESEEEED DESEEDNYVS SAGQRGGQRV VQRGIPRAVA GDEDDEDDVN IDDI

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.