Recombinant D. melanogaster Eukaryotic translation initiation factor 4E(eIF-4E)
- Catalog Number: CDRPO-3110274
- Size: 20 μg; 100 μg; 1000 μg
Target Information
Species | Drosophila melanogaster |
Target Names | eIF-4E |
Alternative Names | eIF4E1; eIF-4E; Eif4e; CG4035; eIF-4F 25 kDa subunit; mRNA cap-binding protein |
Expression Region | 1-259 |
Subcellular Location | Cytoplasm, Cytoplasmic ribonucleoprotein granule. |
Protein Families | Eukaryotic initiation factor 4E family |
Function | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. In 0-1 hour embryos, forms a complex with me31B, cup, tral and pAbp which binds to various mRNAs including maternal mRNAs, and downregulates their expression during the maternal-to-zygotic transition. |
Database Info | KEGG: Dmel_CG4035; STRING: 7227.FBpp0076215; UniGene: Dm.7012 |
Product Details
Product Type | Recombinant Protein |
Source | Yeast; E. coli; Baculovirus; Mammalian cell |
Target Protein Sequence | MQSDFHRMKN FANPKSMFKT SAPSTEQGRP EPPTSAAAPA EAKDVKPKED PQETGEPAGN TATTTAPAGD DAVRTEHLYK HPLMNVWTLW YLENDRSKSW EDMQNEITSF DTVEDFWSLY NHIKPPSEIK LGSDYSLFKK NIRPMWEDAA NKQGGRWVIT LNKSSKTDLD NLWLDVLLCL IGEAFDHSDQ ICGAVINIRG KSNKISIWTA DGNNEEAALE IGHKLRDALR LGRNNSLQYQ LHKDTMVKQG SNVKSIYTL |
Protein Length | Full Length Protein |
Purity | >85% (SDS-PAGE) |
Tag Info | The following tags are available. N-terminal His-tagged Tag-Free |
Form | Lyophilized powder |
Buffer before Lyophilization | Tris/PBS-based buffer, 6% Trehalose, pH 8.0 |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Lead Time | Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times. |
Storage & Handling
Storage Condition | Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use. |
Shelf Life | The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. |
Handling | Repeated freezing and thawing are not recommended. |
Limitations | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week. |
For research use only. Not intended for any clinical use.