Recombinant D. melanogaster Eukaryotic translation initiation factor 3 subunit I(Trip1)

Drosophila melanogaster

eIF3i

eIF3i; eif3-S2; Trip1; CG8882; eIF3i; Eukaryotic translation initiation factor 3 subunit 2; TRIP-1 homolog; eIF-3-beta

1-326

Cytoplasm.

EIF-3 subunit I family

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.

KEGG: Dmel_CG8882; STRING: 7227.FBpp0078689; UniGene: Dm.2654

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MRPLMLQGHE RSITQIKYNR EGDLLFSCSK DQKPNVWYSL NGERLGTYDG HQGAVWCLDV DWESRKLITG AGDMTAKIWD VEYGTVIASI PTKSSVRTSN FSFSGNQAAY STDKAMGQSC ELFLIDVRNA DSSLSEQEPT LRIPMTESKI TSMLWGPLDE TIITGHDNGN IAIWDIRKGQ KVVDSGTDHS AGINDMQLSK DGTMFVTASK DTTAKLFDSE SLMCLKTYKT ERPVNSAAIS PIMDHVVLGG GQDAMEVTTT STKAGKFDSR FFHLIYEEEF ARLKGHFGPI NSLAFHPDGK SYASGGEDGF VRVQTFDSTY FENIFE

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.