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Recombinant D. melanogaster Eukaryotic translation initiation factor 3 subunit I(Trip1)

  • Catalog Number:  CDRPO-3110273
  • Size:   20 μg; 100 μg; 1000 μg

Target Information

Species

Drosophila melanogaster

Target Names

eIF3i

Alternative Names

eIF3i; eif3-S2; Trip1; CG8882; eIF3i; Eukaryotic translation initiation factor 3 subunit 2; TRIP-1 homolog; eIF-3-beta

Expression Region

1-326

Subcellular Location

Cytoplasm.

Protein Families

EIF-3 subunit I family

Function

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.

Database Info

KEGG: Dmel_CG8882; STRING: 7227.FBpp0078689; UniGene: Dm.2654

Product Details

Product Type

Recombinant Protein

Source

Yeast; E. coli; Baculovirus; Mammalian cell

Target Protein Sequence

MRPLMLQGHE RSITQIKYNR EGDLLFSCSK DQKPNVWYSL NGERLGTYDG HQGAVWCLDV DWESRKLITG AGDMTAKIWD VEYGTVIASI PTKSSVRTSN FSFSGNQAAY STDKAMGQSC ELFLIDVRNA DSSLSEQEPT LRIPMTESKI TSMLWGPLDE TIITGHDNGN IAIWDIRKGQ KVVDSGTDHS AGINDMQLSK DGTMFVTASK DTTAKLFDSE SLMCLKTYKT ERPVNSAAIS PIMDHVVLGG GQDAMEVTTT STKAGKFDSR FFHLIYEEEF ARLKGHFGPI NSLAFHPDGK SYASGGEDGF VRVQTFDSTY FENIFE

Protein Length

Full Length Protein

Purity

>85% (SDS-PAGE)

Tag Info

The following tags are available. N-terminal His-tagged Tag-Free

Form

Lyophilized powder

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Lead Time

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Storage & Handling

Storage Condition

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

Shelf Life

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Handling

Repeated freezing and thawing are not recommended.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.

For research use only. Not intended for any clinical use.