Recombinant D. melanogaster Eukaryotic translation initiation factor 3 subunit F-2(eIF3-S5-2)
- Catalog Number: CDRPO-3110272
- Size: 20 μg; 100 μg; 1000 μg
Target Information
Species | Drosophila melanogaster |
Target Names | eIF3f2 |
Alternative Names | eIF3f2; eIF3-S5-2; CG8335; Eukaryotic translation initiation factor 3 subunit F-2; eIF3f-2; Eukaryotic translation initiation factor 3 subunit 5-2 |
Expression Region | 1-285 |
Subcellular Location | Cytoplasm. |
Protein Families | EIF-3 subunit F family |
Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. |
Database Info | KEGG: Dmel_CG8335; STRING: 7227.FBpp0085334; UniGene: Dm.17868 |
Product Details
Product Type | Recombinant Protein |
Source | Yeast; E. coli; Baculovirus; Mammalian cell |
Target Protein Sequence | MSRNFSMRAK VYLKPLVFFQ IIDAYDRRPK GDNQVMGTLL GRNKEGHIEI TNCFTVPHKE HSENKRIDLD MAYASEVLEL NMFAYPNERV LGWFCTGKSV SRSASLIHDY YVRECCEGQP LHLLVDAALK NQRLSTRLYC AVEMGVPGGT KGLMFSLVPL EISNENSDLV ALRCIEKQSQ QQASKQMERF VPELAQVVDA TRDMQHRLDL VLRYINDVLA RKKKPDNVVG RSLYAALTAV PLLDSDKFRV MFNTNLRDML MAITLSTMIK TQLEISEKLS CMQDQ |
Protein Length | Full Length Protein |
Purity | >85% (SDS-PAGE) |
Tag Info | The following tags are available. N-terminal His-tagged Tag-Free |
Form | Lyophilized powder |
Buffer before Lyophilization | Tris/PBS-based buffer, 6% Trehalose, pH 8.0 |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Lead Time | Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times. |
Storage & Handling
Storage Condition | Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use. |
Shelf Life | The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. |
Handling | Repeated freezing and thawing are not recommended. |
Limitations | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week. |
For research use only. Not intended for any clinical use.