Recombinant D. melanogaster ETS-like protein pointed, isoform P2/D(pnt), partial

Drosophila melanogaster

pnt

pnt; DMPOINT1A; ETS2; Ets58AB; pointed; CG17077; ETS-like protein pointed

Nucleus.

ETS family

ETS transcription factor with a prominent role during development of the eye and the nervous system. Required for glial-neuronal cell interactions at the ventral midline which are necessary for the proper elaboration of commissures in the embryonic CNS.; Required for normal EGFR-induced photoreceptor development probably as a downstream effector of Ras85D. In larval eye imaginal disks, activated by MAPK phosphorylation following EGFR activation, induces transcription of isoform P1 which in turn activates transcription of target genes essential for photoreceptor development.; Required for normal EGFR-induced photoreceptor development. Following transcriptional activation by isoform P2, acts as a constitutive activator of transcription, leading to induction of target genes essential for photoreceptor development. In larval brains, involved in the maintenance of type II neuroblast self-renewal and identity together with brat, btd and pros; prevents intermediate neuronal progenitor (INP) dedifferentiation by regulating the expression of erm probably via Notch signaling; suppresses Ase expression in type II neuroblasts and promotes the generation of intermediate neuronal progenitors.

KEGG: Dmel_CG17077; STRING: 7227.FBpp0088658; UniGene: Dm.2370

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

Partial

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.