Recombinant D. melanogaster Elongation factor 1-alpha 1(Ef1alpha48D)

Drosophila melanogaster

Ef1alpha48D

eEF1alpha1; Ef1alpha48D; EF1B; F1; CG8280; EF-1-alpha-1; 50 kDa female-specific protein

1-463

Cytoplasm.

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-Tu/EF-1A subfamily

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

KEGG: Dmel_CG8280; STRING: 7227.FBpp0304260; UniGene: Dm.29457

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MGKEKIHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAQEMG KGSFKYAWVL DKLKAERERG ITIDIALWKF ETAKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGT GEFEAGISKN GQTREHALLA FTLGVKQLIV GVNKMDSSEP PYSEARYEEI KKEVSSYIKK IGYNPAAVAF VPISGWHGDN MLEPSTNMPW FKGWKVERKE GNADGKTLID ALDAILPPAR PTDKALRLPL QDVYKIGGIG TVPVGRVETG VLKPGTVVVF APANITTEVK SVEMHHEALQ EAVPGDNVGF NVKNVSVKEL RRGYVAGDSK ANPPKGAADF TAQVIVLNHP GQIANGYTPV LDCHTAHIAC KFAEIKEKVD RRSGKTTEEN PKFIKSGDAA IVNLVPSKPL CVEAFQEFPP LGRFAVRDMR QTVAVGVIKA VNFKDASGGK VTKAAEKATK GKK

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.