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Recombinant D. melanogaster DEAD-box helicase Dbp80(Dbp80)

  • Catalog Number:  CDRPO-3110211
  • Size:   20 μg; 100 μg; 1000 μg

Target Information

Species

Drosophila melanogaster

Target Names

Dbp80

Alternative Names

Dbp80; Hel40; HEL80; CG17023; DEAD-box helicase Dbp80; EC 3.6.4.13

Expression Region

1-460

Subcellular Location

Cytoplasm. Nucleus, nucleoplasm.

Protein Families

DEAD box helicase family, DDX19/DBP5 subfamily

Function

ATP-dependent RNA helicase involved in mRNA export from the nucleus.

Database Info

STRING: 7227.FBpp0304166

Product Details

Product Type

Recombinant Protein

Source

Yeast; E. coli; Baculovirus; Mammalian cell

Target Protein Sequence

MGDWAKKSED QEVSKLVDKL NLDSKSGEET DFDVADPAET SLLIKILGKG LVNTKLSLDL QQKNPNSPLH SVKTFEALHL KASLLKGIYA MGFNTPSKIQ ETALPTLLAD PPQNMIAQSQ SGTGKTAAFV LAMLSRVNVC LNHPQVLCLS PTYELAIQTG EVAARMGQFC REIKLRFAVR GEEVDRSKKI EEHILIGTPG KLLDWGIKFR LFDMKKISVF VLDEADVMIA TQGHHDQCIR IHKMLNPHCQ MLFFSATYGK EVMDFARLIV ADPTIIRLMR EEESLENIKQ YYVKCKNEEG KYNAIQNIYG CISVGQAIIF CHTKRTAAWL AAKMTSDGHS VAVLTGDLTV VQRLDVLDRF RSGLEKVLIT TNILSRGIDI EQLQVVVNFD LPVDLDGMAD CETYLHRIGR TGRFGKSGIA INLITDEKTM KVCSDIEKHF NKKIEVLNTD SADDIEKIGT

Protein Length

Full Length Protein

Purity

>85% (SDS-PAGE)

Tag Info

The following tags are available. N-terminal His-tagged Tag-Free

Form

Lyophilized powder

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Lead Time

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Storage & Handling

Storage Condition

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

Shelf Life

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Handling

Repeated freezing and thawing are not recommended.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.

For research use only. Not intended for any clinical use.