Recombinant D. melanogaster Cofilin/actin-depolymerizing factor homolog(tsr)

Drosophila melanogaster

tsr

tsr; Cadf; CG4254; Protein D61; Protein twinstar

1-148

Cytoplasm, cytoskeleton. Nucleus matrix.

Actin-binding proteins ADF family

Exhibits F-actin depolymerizing activity and regulates actin cytoskeleton dynamics. Required for cytokinesis in both mitotic and meiotic cells and for aster migration and separation. Promotes cell motility during ovary development and oogenesis. During larval development, required for the cell rearrangement needed for formation of terminal filaments which are stacks of somatic cells that are important for the initiation of ovarioles. Also required for border cell migration during oogenesis. During border cell migration, required for actin turnover and lamellipodial protrusion. Required for the establishment of planar cell polarity (PCP) where cells adopt a uniform orientation within the plane of an epithelium. During establishment of PCP, required for the redistribution of the PCP core proteins fz and stan/fmi to the proximodistal cell boundary. During pupal development, required for elongation of the retinal cell body and for rhabdomere morphogenesis. Required for mushroom body neuroblast proliferation and axon growth. Plays a role in the positive regulation of protein secretion. Plays a role in the regulation of nuclear localization of actin. Required for the maintenance of epithelial integrity by controlling cell junctions and is also necessary for cell survival and tissue growth through regulation of JNK and yki signaling.

KEGG: Dmel_CG4254; STRING: 7227.FBpp0072097; UniGene: Dm.763

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MASGVTVSDV CKTTYEEIKK DKKHRYVIFY IRDEKQIDVE TVADRNAEYD QFLEDIQKCG PGECRYGLFD FEYMHQCQGT SESSKKQKLF LMSWCPDTAK VKKKMLYSSS FDALKKSLVG VQKYIQATDL SEASREAVEE KLRATDRQ

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.