Recombinant D. melanogaster Aurora kinase B(ial)
- Catalog Number: CDRPO-3110441
- Size: 20 μg; 100 μg; 1000 μg
Target Information
| Species | Drosophila melanogaster |
| Target Names | ial |
| Alternative Names | ial; CG6620; Aurora kinase B; EC 2.7.11.1; IPL1/Aurora-like protein kinase; Serine/threonine-protein kinase Ial; Serine/threonine-protein kinase aurora-B |
| Expression Region | 1-329 |
| Subcellular Location | Chromosome. Cytoplasm, cytoskeleton. Midbody. Note=Meiotic and mitotic chromosomes. During each division, relocates to the midbody microtubules. |
| Protein Families | Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily |
| Function | Serine/threonine-protein kinase which mediates both meiotic and mitotic chromosome segregation. Required for histone H3 'Ser-10' phosphorylation. Phosphorylates mei-S332 within residues 124-126 and stabilizes its association with centromeres during meiosis. |
| Database Info | KEGG: Dmel_CG6620; STRING: 7227.FBpp0079769; UniGene: Dm.3103 |
Product Details
| Product Type | Recombinant Protein |
| Source | Yeast; E. coli; Baculovirus; Mammalian cell |
| Target Protein Sequence | MTLSRAKHAN RNHLPHLLAK VPEEHQEPIK NMCLKMMSHD AYGQPYDWSP RDFEMGAHLG RGKFGRVYLA RERHSHYLVA MKVMFKEELR KGCVQRQVLR EIEIQSRLKH PHILRLLTWF HDESRIYLAL EIASEGELFK HLRGAPNHRF DEPRSAKYTY QVANALNYCH LNNVIHRDLK PENILLTSTD DLKLADFGWS AHTPNNKRRT LCGTLDYLPP EMVDGNSYDD SVDQWCLGIL CYEFVVGCPP FESNSTESTY SKIRRMEISY PSHLSKGCKE LIGGLLRKES KGRITLVDVM THYWVKAGMA ERELQLQKRE RGKENTARN |
| Protein Length | Full Length Protein |
| Purity | >85% (SDS-PAGE) |
| Tag Info | The following tags are available. N-terminal His-tagged Tag-Free |
| Form | Lyophilized powder |
| Buffer before Lyophilization | Tris/PBS-based buffer, 6% Trehalose, pH 8.0 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Lead Time | Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times. |
Storage & Handling
| Storage Condition | Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use. |
| Shelf Life | The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. |
| Handling | Repeated freezing and thawing are not recommended. |
| Limitations | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week. |
For research use only. Not intended for any clinical use.