Recombinant D. melanogaster Arginyl-tRNA--protein transferase 1(Ate1)

Drosophila melanogaster

Ate1

Ate1; Arginyltransferase 1; R-transferase 1; EC 2.3.2.8; Arginine-tRNA--protein transferase 1

1-484

R-transferase family

Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues.

KEGG: Dmel_CG9204; STRING: 7227.FBpp0085627; UniGene: Dm.4513

Recombinant Protein

Yeast; E. coli; Baculovirus; Mammalian cell

MSLSIVSYYG SQQSKCGYCA GANCSLSHGM HAYQLDCRDY QDLIDRGWRR CGYYCYKLRN QETCCPCYTI KCNGLEFKLS KSNKRILRRI NRFLRDGKRE SKPEAGDGDG EADADYAIVA PEVTASEPQP QLPDKSPPVI NVEQVASLAT AQRKPTKQAT AAAVEAPTLG SNKSSLLSSS AAAPISNKPC KKAKQMRLDR RLAKLGDSAS YSTKSLTQEK TLRDFLNTDS ETNKHRLKLR LIHVYDDEFR RTLPQSFALY KKYQISIHND PPKDQDAYKE HLQATPLQNE KPWDGPEMGY GSFHQQYWLD DKLIAVGVID ILPGCVSSVY FFYDPDYSFL SLGTYGSLRE IELVQSLAEK VPSLKYYYMG FYIHSCPKMR YKGKLSPSYL LCPETYEWLP LTDVIRAKLD EHKYQRLNED PAARDVNEFL MEHLDEVKLL LGGRTRTDYK HFRQLRGTVS DDDIIIEYSK LVGKECARRM LYVK

Full Length Protein

>85% (SDS-PAGE)

The following tags are available. N-terminal His-tagged Tag-Free

Lyophilized powder

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Delivery time may differ from different purchasing ways or locations, please kindly consult us for specific delivery times.

Store at -20 °C/-80 °C upon receipt, aliquoting is necessary for multiple use.

The shelf life is related to many factors, including storage state, buffer ingredients, storage temperature, and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C.

Repeated freezing and thawing are not recommended.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Store working aliquots at 4 °C for up to one week.