Drosophila Transposon-mediated Transgenesis Service
CD BioSciences is a global research-oriented company, devoting in life science solutions. We rely on comprehensive Drosophila genetic transformation technology platform to tailor-design and build Drosophila transposon systems for customers, and solve the challenges in genetic manipulation for you. We are your loyal partner, escorting your scientific research projects, so that you don't need to be distracted to focus on your research.
DNA Transposons in Drosophila Transgenesis
DNA transposons are mobile genetic elements found in the genomes of various organisms, including Drosophila. They can change their positions within the genome through a cut-and-paste mechanism. DNA transposons in Drosophila Transgenesis play a crucial role in genetic manipulation.
The mechanism involves two main components: the transposon DNA and the transposase enzyme. The transposon contains inverted terminal repeats (ITRs) at both ends, recognized by the transposase. The enzyme recognizes these ITRs, cuts the transposon out of its original position, and then catalyzes its insertion into a new location in the genome. This process allows the transposon to transpose itself or nearby DNA sequences.
Fig.1 The MiMIC transposon system. (Venken KJ., et al. 2011)
In Drosophila Transgenesis, researchers have harnessed DNA transposons like P-element, Minos, and PiggyBac to efficiently introduce foreign genes or modify the host genome. By incorporating the desired gene of interest between the ITRs, the transposase enzyme can help integrate the gene into the Drosophila genome stably and inheritably, making it an essential tool for genetic studies and biotechnology applications.
Our Services
DNA transposons have revolutionized the field of transgenesis by enabling the efficient insertion and stable integration of genes into the Drosophila genome. Our team of experienced scientists and researchers are well-versed in working with these transposon systems, ensuring accurate and reliable results for your research projects. Here's a brief overview of our customized transgenesis services based on DNA transposons:
| Systems | Description | Advantages | Limitations |
|---|---|---|---|
| P-elements | Contain 31 base pairs, including two terminal inverted repeat sequences and transposase; strongly biased towards the 5' end of the gene | Mediate insertion and gene mutations; | Cannot be widely used by other species except Drosophila; few insertions happening in introns |
| PiggyBac Transposons | Contains 13 base pairs of 5' Piggy and 3' Piggy inverted transposon repeats; preferentially targets TAA sequences | Widely used to generate genome-wide genome transposon collections | Exist some degree of integration preference; cannot mediate mutations |
| Minos Transposons | A Tc1/Mariner-like transposon with 255 base pairs of left (MiL) and right (MiR) reverse transposon repeats; almost no sequence preference | Insertions occurred preferentially in introns in Drosophila; Minos-mediated integration cassette (MiMIC) has spectrum applications | 6 bp footprint left after excision |
Why choose Us?
Expertise
Our team comprises highly skilled scientists and researchers with extensive experience in Drosophila research and transgenesis techniques. You can trust us to deliver accurate and reliable results.
Quality Control
We adhere to strict quality control measures throughout the transgenesis process to ensure the production of high-quality transgenic flies. We employ rigorous screening techniques to verify the presence and stability of transgenes.
Timely Delivery
We value your time and understand the importance of meeting project deadlines. Our efficient workflow and dedicated team enable us to deliver results in a timely manner, allowing you to move forward with your research without delays.
Confidentiality
We prioritize the confidentiality of your research and data. Your project details and results are treated with the utmost confidentiality and security, ensuring that your intellectual property remains protected.
Want to Learn More?
CD BioSciences specialize in providing cutting-edge services in the field of Drosophila research. By choosing our customized transgenesis services based on DNA transposons, you can leverage the power of these versatile tools to advance your Drosophila research. Contact us today to discuss your project requirements and discover how we can assist you in achieving your scientific goals.
References
- Venken K J T, Schulze K L, Haelterman N A, et al. MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature methods, 2011, 8(9): 737-743.
- Venken KJ.; et al. MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nat Methods, 2011, 8(9):737-43.
For research use only. Not intended for any clinical use.
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