Anti-D. melanogaster IRE1 alpha [p Ser724] Polyclonal Antibody - BSA Free

ERN1

42358

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and upregulates ER molecular chaperones in order to cope with ER stress. UPR is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha). IRE1 alpha is correlated with X-box binding protein (XBP1) as a potent UPR transcriptional activator. Transcriptional activation of UPR-responsive genes are regulated by the ATF6 and IRE1-XBP1 pathways, which are often regulated by HIFs and contribute to cell survival under ER hypoxic stress. UPR signaling can a) inhibit protein translation to restore cell function, b) activate signaling to increase production of molecular chaperones for protein folding, and c) initiate ubiquitination signaling that leads to degradation of misfolded proteins in ER. IRE1 alpha acts as the sensor of unfolded proteins in the ER. IRE1 alpha not only promotes cell survival but can initiate apoptosis when accumulation of unfolded proteins in the ER causes stress. IRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. When detecting phospho-IRE1 alpha, it is recommended to normalize its band intensity with total IRE1 alpha.

Rabbit

Polyclonal

IgG

Non-conjugated

Immunogen affinity purified

This IRE1 alpha [p Ser724] antibody was raised against a synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein.

Liquid

PBS, pH 7.4

1.0 mg/mL

WB, ChIP, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD

Drosophila melanogaster

Store at -20 °C or -80 °C.

Avoid freeze-thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.