Anti-D. melanogaster Baboon Polyclonal Antibody

Baboon

Transmembrane receptor Patched regulates several developmental processes in both invertebrate and vertebrates. In higher vertebrates the patched (Ptc) also acts as tumor suppressor. The patched receptor signaling involves negatively regulating a GPCR, smoothened. The binding Hedgehog ligand to Ptc, removes the negative interaction of Ptc and allows the signaling by smoothened. It has been shown that Ptc D. melanogasater head development. During head morphogenesis in D. melanogaster Ptc positively interacts with smoothened, which leads to stimulation of Activin type 1 receptor Baboon and activation of cell proliferation in the eye-antennal disc. The expression of activated Baboon in the Ptc domain in Ptc mutant background completely abolish the head developmental defects. Baboon is a 601 amino acid long (Mr. Wt. 73 kDa) protein with a single transmembrane domain. Novus International Inc. has produced epitope specific anti-Baboon antibodies in rabbit. The Anti-Baboon-selective antibodies were generated against a peptide sequence near the C-terminal end of the protein that are unique to Baboon protein. The Baboon-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. The Baboon antibodies label a 73-74 kDa protein in D. melanogaster extracts. Anti-Baboon-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses.

Rabbit

Polyclonal

IgG

Non-conjugated

Immunogen affinity purified

Synthetic peptide: QPDPSIEEMKKVVCIEKCRPN, corresponding to amino acids 552 to 572 of Baboon.The was generated against a peptide sequence near the C terminal end of the protein.The peptides was amidated for conjugation.

Liquid

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

0.75 mg/mL

WB, IP

Drosophila melanogaster

Store at -20 °C or -80 °C.

Avoid freeze-thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.