Anti-D. melanogaster alpha Tubulin Monoclonal Antibody (DM1A) - BSA Free

TUBA1A

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications. Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence.

Mouse

Monoclonal

IgG1 Kappa

Non-conjugated

Protein G purified

This alpha Tubulin Antibody (DM1A) was developed against native chicken brain microtubules.

Liquid

PBS, pH 7.4

1.0 mg/mL

WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, CyTOF-ready

Drosophila melanogaster

Store at -20 °C or -80 °C.

Avoid freeze-thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.